Target (protein/gene name): Transketolase NCBI Gene # or RefSeq#: 3266276 Protein ID (NP or XP #) or Wolbachia#: Q5GRX2 Organism: Wolbachia Etiologic Risk Group (see link below): n/a Background/Disease Information: Essentiality of this protein: (non essential in two studies with e. coli, essential in a study with tuberculosis) http://www.tdrtargets.org/targets/view?gene_id=232798 Complex of enzymes: EC#: 2.2.1.1 Link to BRENDA EC# page: http://www.brenda-enzymes.org/php/result_flat.php4?ecno=2.2.1.1
Structure Available (PDB or Homology model) -- PDB # or closest PDB entry if using homology model: Campylobacter jejuni 3M34
-- Query Coverage (if not direct match): 98% -- Max Ident (if not direct match): 50% Druggable Target (see Databases for this): http://www.tdrtargets.org/targets/view?gene_id=232798 Current Inhibitors: Expression Information (has it been expressed in bacterial cells): Purification Method: Image of protein (PyMol or etc): Amino Acid Sequence:
length of your protein in Amino Acids Molecular Weight of your protein in kiloDaltons using the Expasy ProtParam website Molar Extinction coefficient of your protein at 280 nm wavelength: CDS Gene Sequence: GC% Content for gene: CDS Gene Sequence (codon optimized) - after having done Primer Design: GC% Content for gene (codon optimized):
Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):
Primer design results for 'tail' primers (this is just 2 sequences):
NCBI Gene # or RefSeq#: 3266276
Protein ID (NP or XP #) or Wolbachia#: Q5GRX2
Organism: Wolbachia
Etiologic Risk Group (see link below): n/a
Background/Disease Information:
Essentiality of this protein: (non essential in two studies with e. coli, essential in a study with tuberculosis)
http://www.tdrtargets.org/targets/view?gene_id=232798
Complex of enzymes:
EC#: 2.2.1.1
Link to BRENDA EC# page:
http://www.brenda-enzymes.org/php/result_flat.php4?ecno=2.2.1.1
Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):
-- link to Sigma (or other company) page for assay or assay reagents (substrates)
http://www.sigmaaldrich.com/catalog/search?interface=All&term=D-ribose%205-phosphate&lang=en®ion=US&focus=product&N=0+220003048+219853269+219853286
http://www.sigmaaldrich.com/catalog/search?interface=All&term=D-xylulose%205-phosphate&lang=en®ion=US&focus=product&N=0+220003048+219853269+219853286
D-xylulose 5-phosphate + D-ribose 5-phosphate available on sigma
-- link to paper that contains assay information
http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Enzyme_Assay/transketolase.Par.0001.File.tmp/transketolase.pdf
Structure Available (PDB or Homology model)
-- PDB # or closest PDB entry if using homology model:
Campylobacter jejuni 3M34
-- Query Coverage (if not direct match): 98%
-- Max Ident (if not direct match): 50%
Druggable Target (see Databases for this):
http://www.tdrtargets.org/targets/view?gene_id=232798
Current Inhibitors:
Expression Information (has it been expressed in bacterial cells):
Purification Method:
Image of protein (PyMol or etc):
Amino Acid Sequence:
<span style="background-color: #f5f5f5; text-align: left;"> 1**__0__** 2**__0__** 3**__0__** 4**__0__** 5**__0__** 6**__0__** MSSRKELANA IRALSMDAVQ KAKSGHPGAP MGMADIAEVL WRDFLKHNPQ NPSWADRDRF 7**__0__** 8**__0__** 9**__0__** 10**__0__** 11**__0__** 12**__0__** VLSNGHGSML IYSLLHLTGY DLPMEELKNF RQLHSKTPGH PEVGYTAGVE TTTGPLGQGI 13**__0__** 14**__0__** 15**__0__** 16**__0__** 17**__0__** 18**__0__** ANAVGMAIAE KTLAAQFNRP GHDIVDHYTY AFMGDGCMME GISHEVCSLA GTLKLGKLIA 19**__0__** 20**__0__** 21**__0__** 22**__0__** 23**__0__** 24**__0__** FYDDNGISID GHVEGWFTDD TAMRFEAYGW HVIRDIDGHD AASIKRAVEE ARAVTDKPSL 25**__0__** 26**__0__** 27**__0__** 28**__0__** 29**__0__** 30**__0__** LMCKTIIGFG SPNKAGTHDS HGAPLGDAEI ALTREQLGWK YAPFEIPSEI YAQWDAKEAG 31**__0__** 32**__0__** 33**__0__** 34**__0__** 35**__0__** 36**__0__** QAKESAWNEK FAAYAKAYPQ EAAEFTRRMK GEMPSDFDAK AKEFIAKLQA NPAKIASRKA 37**__0__** 38**__0__** 39**__0__** 40**__0__** 41**__0__** 42**__0__** SQNAIEAFGP LLPEFLGGSA DLAPSNLTLW SGSKAINEDA AGNYIHYGVR EFGMTAIANG 43**__0__** 44**__0__** 45**__0__** 46**__0__** 47**__0__** 48**__0__** ISLHGGFLPY TSTFLMFVEY ARNAVRMAAL MKQRQVMVYT HDSIGLGEDG PTHQPVEQVA 49**__0__** 50**__0__** 51**__0__** 52**__0__** 53**__0__** 54**__0__** SLRVTPNMST WRPCDQVESA VAWKYGVERQ DGPTALILSR QNLAQQERTE EQLANIARGG 55**__0__** 56**__0__** 57**__0__** 58**__0__** 59**__0__** 60**__0__** YVLKDCAGQP ELIFIATGSE VELAVAAYEK LTAEGVKARV VSMPSTDAFD KQDAAYRESV 61**__0__** 62**__0__** 63**__0__** 64**__0__** 65**__0__** 66**__0__** LPKAVTARVA VEAGIADYWY KYVGLNGAIV GMTTFGESAP AELLFEEFGF TVDNVVAKAK ELL </span>length of your protein in Amino Acids
Molecular Weight of your protein in kiloDaltons using the Expasy ProtParam website
Molar Extinction coefficient of your protein at 280 nm wavelength:
CDS Gene Sequence:
GC% Content for gene:
CDS Gene Sequence (codon optimized) - after having done Primer Design:
GC% Content for gene (codon optimized):
Primer design results for pNIC-Bsa4 cloning (list seqeunces of all of your ~40 nt long primers):
Primer design results for 'tail' primers (this is just 2 sequences):